AM32049SU-N Major Vault Protein antibody

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1 ml / €430.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Human Major Vault Protein LMR5

AM32049SU-N

Product Description for Major Vault Protein

Rat anti Human Major Vault Protein LMR5.
Presentation: Liquid
Product is tested for Paraffin Sections, Frozen Sections.

Properties for Major Vault Protein

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms LRP, Lung-Resistance Related Protein, MVP
Presentation Liquid
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone LMR5
Host Rat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q14764
GeneID:
9961
Add. information

Mab producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized mouse (Balb/c) with SP2/O mouse myeloma cells.
Culture Medium: RPMI-1640 (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-SR (Boehringer, Indianapolis, USA). The medium does not contain serum nor added enzymes. The antibody solution has been filtered through a 0.22 micron filter.
NOTE: This monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured.

Application Flow Cytometry (Cell permeabilization required).
Immunohistochemistry on Frozen Sections, Air-Dried or Acetone Fixed Cells: 1/20.
Immunohistochemistry on Paraffin Embedded Sections.
This antibody LMR5 has potential value for detection of LRP/MVP-associated non-Pgp MDR in Human tumor samples.
Background MVP is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Amino acid 241-280 of human estrogen receptor (ER), (site of nuclear localization signal sequence), is mapped to be the site of interaction between ER and MVP. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen 1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy.
This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins.
Concentration ~0.25 mg Ig/ml
General Readings
  1. Scheper RJ, Broxterman HJ, Scheffer GL, Kaaijk P, Dalton WS, van Heijningen TH, et al. Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance. Cancer Res. 1993 Apr 1;53(7):1475-9. PubMed PMID: 7680954.
  2. Scheffer GL, Wijngaard PL, Flens MJ, Izquierdo MA, Slovak ML, Pinedo HM, et al. The drug resistance-related protein LRP is the human major vault protein. Nat Med. 1995 Jun;1(6):578-82. PubMed PMID: 7585126.
  3. Izquierdo MA, van der Zee AG, Vermorken JB, van der Valk P, Beliën JA, Giaccone G, et al. Drug resistance-associated marker Lrp for prediction of response to chemotherapy and prognoses in advanced ovarian carcinoma. J Natl Cancer Inst. 1995 Aug 16;87(16):1230-7. PubMed PMID: 7563169.
  4. Flens MJ, Scheffer GL, van der Valk P, Broxterman HJ, Eijdems EW, Huysmans AC, et al. Identification of novel drug resistance-associated proteins by a panel of rat monoclonal antibodies. Int J Cancer. 1997 Oct 9;73(2):249-57. PubMed PMID: 9335451.
  5. Schroeijers AB, Scheffer GL, Flens MJ, Meijer GA, Izquierdo MA, van der Valk P, et al. Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol. 1998 Feb;152(2):373-8. PubMed PMID: 9466563. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. 
Avoid repeated freezing and thawing. 
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid
Liquid
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody LMR5 reacts with an internal epitope of the LRP/Major Vault Protein (P110), which is stronly overexpressed in various Human non-P-glycoprotein MDR tumor cell lines.
Gene ID 9961

Accessory Products

Proteins and/or Positive Controls

Proteins for Major Vault Protein (5 products)

Catalog No. Species Pres. Purity   Source  

Major Vault Protein (transcript variant 2)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein (transcript variant 1)

Major Vault Protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Major Vault Protein

Major Vault Protein Human
  Abnova Taiwan Corp.

Positive controls for Major Vault Protein (1 products)

Catalog No. Species Pres. Purity   Source  

MVP Lysate

Western Blot: MVP Lysate [NBL1-13402] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for MVP.
  Novus Biologicals Inc.
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