R1151BS Maltose Phosphorylase antibody

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0.1 mg / €440.00
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Goat anti Escherichia coli Maltose Phosphorylase


Product Description for Maltose Phosphorylase

Goat anti Escherichia coli Maltose Phosphorylase.
Presentation: Biotin
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Enzyme Immunoassay.

Properties for Maltose Phosphorylase

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Biotin
Reactivity E. coli
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Maltose Phosphorylase from E.coli
Application Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA: 1/5,000-1/20,000.
This antibody has been assayed against 1.0 µg of Maltose Phosphorylase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Background Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
0.01% (w/v) Sodium azide
10 mg/ml BSA (immunoglobulin and protease free)
Lyophilized purified Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This antibody detects Maltose Phosphorylase [E.coli]. Cross reactivity against Maltose Phosphorylase from other sources is unknown.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Maltose Phosphorylase [E.coli].

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