R1151P Maltose Phosphorylase antibody

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50 mg / €5,440.00

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Goat anti Escherichia coli Maltose Phosphorylase


Product Description for Maltose Phosphorylase

Goat anti Escherichia coli Maltose Phosphorylase.
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Maltose Phosphorylase

Product Category Primary Antibodies
Quantity 50 mg
Presentation Ig Fraction
Reactivity E. coli
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Maltose Phosphorylase [E.coli].
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods requiring high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Maltose Phosphorylase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Background Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Multistep process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
Lyophilized purified Ig fraction.
Ig Fraction
Restore with 5.0 ml of deionized water (or equivalent).
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.coli].
Cross reactivity against Maltose Phosphorylase from other sources is unknown.

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