discontinued

AP03013PU-N MAPKK1 / MAPKK2 pSer218/222 antibody

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Search for all "MAPKK1 / MAPKK2"

50 µg / €200.00

Quick Overview

Rabbit anti Human MAPKK1 / MAPKK2

Product Description for MAPKK1 / MAPKK2

Rabbit anti Human MAPKK1 / MAPKK2.
Properties: pSer218/222
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for MAPKK1 / MAPKK2

Product Category Primary Antibodies
Quantity 50 µg
Synonyms MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2
Presentation Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1.
Remarks:
The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).
Property pSer218/222
Application Western Blotting under reducing and non-reducing conditions:
Recommended dilution: 0.5 μg/ml
Positive material: RAJI human Burkitt lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Reducing conditions are recommended.
Background MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade - an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.
Concentration 1.0 mg/ml
General Readings
  1. Chang F, Steelman LS, Lee JT, Shelton JG, Navolanic PM, Blalock WL, et al. Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: potential targeting for therapeutic intervention. Leukemia. 2003 Jul;17(7):1263-93. PubMed PMID: 12835716.
  2. Park JI, Strock CJ, Ball DW, Nelkin BD. The Ras/Raf/MEK/extracellular signal-regulated kinase pathway induces autocrine-paracrine growth inhibition via the leukemia inhibitory factor/JAK/STAT pathway. Mol Cell Biol. 2003 Jan;23(2):543-54. PubMed PMID: 12509453. (Free PMC Article available, 7 images available)
  3. Sundaram MV: RTK/Ras/MAPK signaling. WormBook. 2006 Feb 11;:1-19.
  4. McCubrey JA, Steelman LS, Chappell WH, Abrams SL, Wong EW, Chang F, et al. Roles of the Raf/MEK/ERK pathway in cell growth, malignant transformation and drug resistance. Biochim Biophys Acta. 2007 Aug;1773(8):1263-84. Epub 2006 Oct 7. PubMed PMID: 17126425. (Free PMC Article available, 7 images available)
  5. Scholl FA, Dumesic PA, Barragan DI, Harada K, Bissonauth V, Charron J, et al. Mek1/2 MAPK kinases are essential for Mammalian development, homeostasis, and Raf-induced hyperplasia. Dev Cell. 2007 Apr;12(4):615-29. PubMed PMID: 17419998.
  6. Park ER, Eblen ST, Catling AD. MEK1 activation by PAK: a novel mechanism. Cell Signal. 2007 Jul;19(7):1488-96. Epub 2007 Jan 24. PubMed PMID: 17314031. (Free PMC Article available, 5 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch
Format
Purification:
Immunoffinity chromatography.
Buffer System:
PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
State:
Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
Purified
Specificity
Specificity:
The polyclonal antibody reacts with MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.
Species:
Human.
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