AP05185PU-N MAPKK1 / MAPKK2 pSer218/222 antibody

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10 Blots / €470.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat, Xenopus MAPKK1 / MAPKK2

Product Description for MAPKK1 / MAPKK2

Rabbit anti Human, Mouse, Rat, Xenopus MAPKK1 / MAPKK2.
Properties: pSer218/222
Presentation: Aff - Purified
Product is tested for Dot blot, Western blot / Immunoblot.

Properties for MAPKK1 / MAPKK2

Product Category Primary Antibodies
Quantity 10 Blots
Synonyms MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt, Xen
Applications Dot, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser218/222 of Human MEK1/2
Property pSer218/222
Application Western Blot: 1/1000 dilution to provide for 10 miniblots.
Dot Blot: 1/1000 dilution to provide for 10 miniblots.
Background MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.
Concentration Lot specific
General Readings 1. Adams JP, Sweatt JD. Molecular psychology: roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol. 2002;42:135-63. PubMed PMID: 11807168.
2. Ahn NG, Robbins DJ, Haycock JW, Seger R, Cobb MH, Krebs EG. Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin. J Neurochem. 1992 Jul;59(1):147-56. PubMed PMID: 1319464.
3. Ahn NG. The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem. 1993 Nov;127-128:201-9. PubMed PMID: 7935352.
4. Crews CM, Alessandrini A, Erikson RL. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science. 1992 Oct 16;258(5081):478-80. PubMed PMID: 1411546.
5. Park SH, Zarrinpar A, Lim WA. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science. 2003 Feb 14;299(5609):1061-4. Epub 2003 Jan 2. PubMed PMID: 12511654
6. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463- 469.
7. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046.
8. Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos- phorylation on both
tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Antigen Immunoaffinity Chromatography
Buffer System:
HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol
Purity:
Aff - Purified
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity. 
Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Species:
Human, Mouse, Rat, Xenopus.

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