AP08687PU-N MAPKK1 / MAPKK2 pSer218/222 antibody

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Rabbit anti Bovine, Canine, Chicken, Human, Mouse, Rat, Xenopus, Zebrafish MAPKK1 / MAPKK2

Product Description for MAPKK1 / MAPKK2

Rabbit anti Bovine, Canine, Chicken, Human, Mouse, Rat, Xenopus, Zebrafish MAPKK1 / MAPKK2.
Properties: pSer218/222
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for MAPKK1 / MAPKK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2
Presentation Aff - Purified
Reactivity Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Xen, Ze
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of Human MEK 1/2.
Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
Property pSer218/222
Application Western blot: 1/1000.
Background MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
General Readings
  1. Adams JP, Sweatt JD. Molecular psychology: roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol. 2002;42:135-63. PubMed PMID: 11807168.
  2. Ahn NG. The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem. 1993 Nov;127-128:201-9. PubMed PMID: 7935352.
  3. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
  4. Crews CM, Alessandrini A, Erikson RL. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science. 1992 Oct 16;258(5081):478-80. PubMed PMID: 1411546.
  5. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J. pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem. 1991 Jun 5;266(16):10043-6. PubMed PMID: 1645334.
  6. Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6142-6. PubMed PMID: 1712480. (Free PMC Article available)
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Buffer System:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Chicken, Human, non-Human Primates, Rat, Xenopus and Zebrafish.
Species reactivity (tested):
This antibody is specific for the ~45k MEK1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

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