AM50292PU-N MART-1 antibody

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0.2 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Canine, Human, Mouse, Rat MART-1 A103 + M2-7C10 + M2-9E3

Product Description for MART-1

Mouse anti Canine, Human, Mouse, Rat MART-1 A103 + M2-7C10 + M2-9E3.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for MART-1

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms Antigen SK29-AA, LB39-AA, MART1, MLANA, Melan-A protein, MelanA, Melanoma antigen recognized by T-cells 1
Presentation Purified
Reactivity Can, Hu, Ms, Rt
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone A103 + M2-7C10 + M2-9E3
Host Mouse
Isotype IgG1/IgG2b
Molecular weight 20-22kDa (doublet)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16655
Immunogen:
Recombinant Human MART-1 protein (A103; M2-7C10; M2-9E3).
GeneID:
2315
Application Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas.
Concentration 0.2 mg/ml
General Readings
  1. Chen YT, Stockert E, Jungbluth A, Tsang S, Coplan KA, Scanlan MJ, et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5915-9. PubMed PMID: 8650193. (Free PMC Article available)
  2. Kawakami Y, Battles JK, Kobayashi T, Ennis W, Wang X, Tupesis JP, et al. Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: use in the characterization of the human melanoma antigen MART-1. J Immunol Methods. 1997 Mar 10;202(1):13-25. PubMed PMID: 9075767.
  3. Marincola FM, Hijazi YM, Fetsch P, Salgaller ML, Rivoltini L, Cormier J, et al. Analysis of expression of the melanoma-associated antigens MART-1 and gp100 in metastatic melanoma cell lines and in in situ lesions. J Immunother Emphasis Tumor Immunol. 1996 May;19(3):192-205. PubMed PMID: 8811494.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Cellular Localization: Cytoplasmic.
Species:
Human, Mouse, Rat and Dog.

Accessory Products

Proteins and/or Positive Controls

Proteins for MART-1 (8 products)

Catalog No. Species Pres. Purity   Source  

MART-1

MART-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MART-1 (48-118, His-tag)

MART-1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

MART-1 (48-118, His-tag)

MART-1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

MART-1

MART-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MART-1

MART-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for MART-1 (1 products)

Catalog No. Species Pres. Purity   Source  

MLANA overexpression lysate

MLANA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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