DM3130 Mast Cell Chymase antibody

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Mouse anti Human Mast Cell Chymase CC1


Product Description for Mast Cell Chymase

Mouse anti Human Mast Cell Chymase CC1.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Mast Cell Chymase

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB2377
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone CC1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Purified Human Skin Chymase
Epitope not determined
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blotting: Ab 1-2 ug/ml for 2 hrs at RT.
Immunohistology (Formalin/paraffin): Use Ab at 0.06-0.12 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended positive control: Tonsil.
Background Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human mast cell chymase is considered to be an important marker of mast cells, as well as, an important mediator of inflammation.
Concentration 0.2 mg/ml
General Readings
  1. Buckley, M.G. et al. (1999). J. Pathol. 189: 138-143.
Storage Store the antibody at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G affinity chromatography
Buffer System:
10mM PBS, pH 7.4, containing 0.2% BSA and 0.09% sodium azide
Liquid Ig fraction
Mast Cell Chymase
Cellular localization: cytoplasmic

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