TA328055 Mast Cell Protease-8 antibody

Rat Monoclonal anti-mMCP-8 Antibody

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0.1 mg / €335.00
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Quick Overview

Rat anti Mouse Mast Cell Protease-8 TUG8

Product Description for Mast Cell Protease-8

Rat anti Mouse Mast Cell Protease-8 TUG8.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Mast Cell Protease-8

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms MCPTT8, mMCP-8
Presentation Purified
Reactivity Ms
Applications WB
Clonality Monoclonal
Clone TUG8
Host Rat
Isotype IgG2a
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Swiss Prot Num:
Recombinant GST-Mouse mMCP-8 fusion protein expressed in E.coli.
Isotype control SM15P, SM15PX
Application Western blot: Each lot of this antibody is quality control tested.
Recommended Dilutions: Use 2.0 µg/ml. 
See also Application References 1,2.
Background mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.
Structure: A 247-aa polypeptide including a signal sequence of 18 aa followed by a 2-aa activation peptide (Gly-Glu) and a mature protease of 227 aa.
 mMCP-8 was initially isolated as a cDNA from a mouse mast cell line, but has recently been found to be expressed primarily by mouse basophils.
Concentration 0.5 mg/ml
General Readings
  1. Ugajin T, Kojima T, Mukai K, Obata K, Kawano Y, Minegishi Y, et al. Basophils preferentially express mouse Mast Cell Protease 11 among the mast cell tryptase family in contrast to mast cells. J Leukoc Biol. 2009 Dec;86(6):1417-25. doi: 10.1189/jlb.0609400. PubMed PMID: 19703899.
  2. Uto-Konomi A, Miyauchi K, Ozaki N, Motomura Y, Suzuki Y, Yoshimura A, et al. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS One. 2012;7(7):e40343. doi: 10.1371/journal.pone.0040343. Epub 2012 Jul 5. PubMed PMID: 22792286. (Free PMC Article available, 7 images available)
  3. Lützelschwab C, Huang MR, Kullberg MC, Aveskogh M, Hellman L. Characterization of mouse mast cell protease-8, the first member of a novel subfamily of mouse mast cell serine proteases, distinct from both the classical chymases and tryptases. Eur J Immunol. 1998 Mar;28(3):1022-33. PubMed PMID: 9541598.
  4. Lunderius C, Hellman L. Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes. Immunogenetics. 2001 Apr;53(3):225-32. PubMed PMID: 11398967.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
PBS, pH 7.2
0.09% Sodium Azide
Liquid purified IgG fraction
This TUG8 monoclonal antibody recognizes Mouse Mast Cell Protease-8 (mMCP-8). Other species not tested.

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