discontinued

NB100-56318 MBD4 antibody

See related secondary antibodies

Search for all "MBD4"

0.1 mg / €350.00

Quick Overview

Rabbit anti Human MBD4

NB100-56318

Product Description for MBD4

Rabbit anti Human MBD4.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for MBD4

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-282 and 337-352 of human MBD4.
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).
Format
Purification:
Affinity Purified
Buffer System:
PBS containing 0.02% sodium azide. Sodium azide is highly toxic
Purity:
Affinity Purified
State:
Aff - Purified
Specificity
Species:
Human Not yet tested in other species.

Accessory Products

  • LinkedIn