discontinued

SP7111P MBD4 antibody

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0.1 mg / €330.00

Quick Overview

Rabbit anti Human MBD4

Product Description for MBD4

Rabbit anti Human MBD4.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for MBD4

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mixture of synthetic peptides (aa 268-282 and 337-352 of hMBD4).
Application Western blot (2 µg/ml).
IF/ICC: 32-64 µg/ml (see Product citation 1).
IHC: 64 µg/ml (see Product citation 1).
Recommended Positive Control: HL60 (a 64 kDa band is observed).
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).
Concentration 0.5 mg/ml
General Readings 1. Bader S, Walker M, Harrison D. Most microsatellite unstable sporadic colorectal carcinomas carry MBD4 mutations. Br J Cancer 83(12): 1646-1649 (2000).
2. Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M. A mammalian protein with specific demethylase activity for mCpG DNA. Nature, 397 (6720):579-583 1999.
3. Hendrich B and Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol, 18: 6538-6547 (1998).
4. Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275 (42): 32422-32429 (2000).
Product Citations
1. THE LOCALIZATION OF A METHYL BINDING DOMAIN PROTEIN (MBD4) IN MURINE AND BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS. N. Ruddock A , B , J. Xue A , B , L. Sanchez-Partida A , B , M. Cooney A , B , N. Korfiatis A , B and M. Holland A , C . Reproduction, feritilty and development, Abstract 2005. (ICC and IHC)
2. Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples. Karen N. Thatcher, Sailaja Peddada, Dag H. Yasui, and Janine M. LaSalle. Hum. Mol. Genet. 14: 785-797 (2005).
Storage Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS containing 0.02% sodium azide
State:
Liquid Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for MBD4.
Species:
Human.

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