AM26335PU-N MBL-A antibody

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0.1 mg / €440.00
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Quick Overview

Rat anti Mouse MBL-A 8G6

Product Description for MBL-A

Rat anti Mouse MBL-A 8G6.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Frozen Sections.

Properties for MBL-A

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ms
Applications C, E, ICC/IF, WB
Clonality Monoclonal
Clone 8G6
Host Rat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Purified mouse MBL-A
Add. information Note that the monoclonal antibody 8G6 is a calcium-dependent antibody.
Application Immunohistochemistry on frozen sections (2,6): Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4⁰C to quench endogenous peroxidases. Primary antibody 8g6, 2µg/ml. As negative control  rat IgG2a was used (Ref.2). The typical starting working dilution is 1:50.
Immunoassays (2).
Immunoflourescence (3,4).
Western blot (1): A non-reduced sample treatment was used. The band sizes are 191, 263 and 316 kDa  (Ref.1). The typical starting working dilution is 1:50.

Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity.
In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen-specific IgM responses and suffer from increased susceptibility to infection.

Concentration 0.1 mg/ml
General Readings
  1. Liu H, Jensen L, Hansen S, Petersen SV, Takahashi K, Ezekowitz AB, et al. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol. 2001 May;53(5):489-97. PubMed PMID: 11309157.
  2. Windbichler M, Echtenacher B, Takahashi K, Ezekowitz RA, Schwaeble WJ, Jenseniuis JC, et al. Investigations on the involvement of the lectin pathway of complement activation in anaphylaxis. Int Arch Allergy Immunol. 2006;141(1):11-23. Epub 2006 Jun 23. PubMed PMID: 16804320.
  3. Held K, Thiel S, Loos M, Petry F. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol. 2008 Sep;45(15):3934-41. doi: 10.1016/j.molimm.2008.06.021. Epub 2008 Jul 30. PubMed PMID: 18672286.
  4. Petry F, Jakobi V, Wagner S, Tessema TS, Thiel S, Loos M. Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to infection. Mol Immunol. 2008 Jul;45(12):3392-400. doi: 10.1016/j.molimm.2008.04.010. Epub 2008 May 23. PubMed PMID: 18501966.
  5. Abe Y, Kuroda Y, Kuboki N, Matsushita M, Yokoyama N, Kojima N. Contribution of complement component C3 and complement receptor type 3 to carbohydrate-dependent uptake of oligomannose-coated liposomes by peritoneal macrophages. J Biochem. 2008 Nov;144(5):563-70. doi: 10.1093/jb/mvn101. Epub 2008 Aug 11. PubMed PMID: 18694897.
  6. Matthijsen RA, de Winther MP, Kuipers D, van der Made I, Weber C, Herias MV, et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation. 2009 Apr 28;119(16):2188-95. doi: 10.1161/CIRCULATIONAHA.108.830661. Epub 2009 Apr 20. PubMed PMID: 19380618.
Protein G
Buffer System:
0.02% sodium azide
0.1% bovine serum albumin
Liquid 0.2 µm filtered Ig fraction
Species Reactivity
Species reactivity (tested):
This antibody detects MBL-A.

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