AB0040-200 mCherry antibody

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0.6 mg / €320.00
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Quick Overview

Goat anti mCherry

Product Description for mCherry

Goat anti mCherry.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for mCherry

Product Category Primary Antibodies
Quantity 0.6 mg
Synonyms Cherry Fluorescent Protein, Red Fluorescent Protein
Presentation Aff - Purified
Applications C, ICC/IF, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer SICGEN

Datasheet Extract

Immunogen
Immunogen:
Purified recombinant peptide produced in E. coli.
Application Western blot: 1/500-1/2,000. 
Immunofluorescence: 1/50-1/500. 
Immunohistochemistry on Paraffin Sections: 1/50-1/500.
Immunohistochemistry on Frozen Sections: 1/50-1/500.
Background mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically "for the discovery and development of the green fluorescent protein, GFP". On expression from the GFP gene, GFP protein will fold correctly and fluoresce strongly, the development of fluoresence requires no cofactors except molecular oxygen. It can therefore be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell under aerobic conditions. As a result DNA encoding GFP can be fused to DNA encoding other proteins as a means to visualize the resulting fusion protein in live cells or animals. Engineered forms of GFP and relatives have been developed to monitor Calcium levels, protease activation and in a variety of other processes in real time. A whole range of GFP derivatives with different spectral properties have been developed, largely in the Tsien lab. The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome.
Concentration 3.0 mg/ml
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Epitope Affinity Chromatography
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
20% Glycerol
State:
Liquid purified IgG fraction
Aff - Purified
Specificity
Specificity:
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody (AB0040) recognizes very well tdTomato and does not recognize GFP (green fluorescent protein).

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