BM2043 Melanoma Associated Antigen (25-110 kDa) antibody

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Mouse anti Human Melanoma Associated Antigen NKI/C3


Product Description for Melanoma Associated Antigen

Mouse anti Human Melanoma Associated Antigen NKI/C3.
Properties: (25-110 kDa)
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Melanoma Associated Antigen

Product Category Primary Antibodies
Quantity 1 ml
Presentation Ascites
Reactivity Hu
Applications C, E, ICC/IF, P, WB
Clonality Monoclonal
Clone NKI/C3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isolated from Human Melanoma cells.
Property 25-110 kDa
Application ELISA.
Immunohistochemistry on Paraffin Sections.
Working Dilution: 1/5-1/10.
Dilution Buffer: Dilute immediately before use with PBS.
Incubation Time: 1h at RT.
Recommended Positive Control: Melanoma.
General Readings
  1. van Duinen SG, Ruiter DJ, Hageman P, Vennegoor C, Dickersin GR, Scheffer E, et al. Immunohistochemical and histochemical tools in the diagnosis of amelanotic melanoma. Cancer. 1984 Apr 1;53(7):1566-73. PubMed PMID: 6365305.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Liquid Ascites
Species Reactivity
Species reactivity (tested):
NKI/C-3 recognizes a melanoma-associated antigen present in the cytoplasm of melanoma cells and nevocellular nevi, in contrast to most mabs against melanoma-associated antigens present in the membrane, which are not retained after formalin fixation. Therefore, the mab is very useful for paraffin sections in routine pathology.
Cross reactivity with carcinoids, medullary carcinomas of the thyroid, and occasionally prostatic, primary breast, avarian, and lung carcinomas may be observed.
No reaction is seen with basal cell carcinomas or brain tumors. NKI/C-3 does not react with normal melanocytes or other normal tissues except for mastcells, histiocytes, salivary glands, bronchial glands, pancreatic und prostatic epithelium. In immunoblotting the antibody reacts with diffuse protein bands between 25 and 100 kD.

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