discontinued

AM50295PU-S Melanoma Marker (MART-1 + gp100) antibody

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Mouse anti Human Melanoma Marker (MART-1 + gp100) DT101+ BC199 + HMB45

Product Description for Melanoma Marker (MART-1 + gp100)

Mouse anti Human Melanoma Marker (MART-1 + gp100) DT101+ BC199 + HMB45.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Melanoma Marker (MART-1 + gp100)

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms MLAN-A, Melanoma antigen recognized by T-cells 1 (MART-1), PMEL17
Presentation Purified
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone DT101+ BC199 + HMB45
Host Mouse
Isotype IgG1/IgG2b
Molecular weight 20-22kDa (doublet) (MART-1); 90-100kDa (gp100)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16655
Immunogen:
Recombinant Human MART-1 protein (Mouse IgG2b, DT101& BC199) and Extract of pigmented melanoma metastases from lymph nodes (Mouse IgG1, HMB45).
GeneID:
2315
Application Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas.
Concentration 0.2 mg/ml
General Readings
  1. Chen YT, Stockert E, Jungbluth A, Tsang S, Coplan KA, Scanlan MJ, et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5915-9. PubMed PMID: 8650193. (Free PMC Article available)
  2. Chen YT, Stockert E, Tsang S, Coplan KA, Old LJ. Immunophenotyping of melanomas for tyrosinase: implications for vaccine development. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8125-9. PubMed PMID: 7667256. (Free PMC Article available)
  3. Gown AM, Vogel AM, Hoak D, Gough F, McNutt MA. Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol. 1986 May;123(2):195-203. PubMed PMID: 3518473. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Cellular Localization: Cytoplasmic.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Melanoma Marker (MART-1 + gp100) (8 products)

Catalog No. Species Pres. Purity   Source  

MART-1

MART-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MART-1 (48-118, His-tag)

MART-1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MART-1 (48-118, His-tag)

MART-1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  Acris Antibodies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  Acris Antibodies GmbH

MART-1

MART-1 Human Purified > 90 % E. coli
  Acris Antibodies GmbH

MART-1

MART-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MART-1

MART-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Melanoma Marker (MART-1 + gp100) (1 products)

Catalog No. Species Pres. Purity   Source  

MLANA overexpression lysate

MLANA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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