AP31114SU-N mGluR2/3 antibody

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Rabbit anti Mouse, Rat mGluR2/3


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  • AP31114SU-N

Product Description for mGluR2/3

Rabbit anti Mouse, Rat mGluR2/3.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for mGluR2/3

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms GRM2, GRM3, Metabotropic glutamate receptor 2, Metabotropic glutamate receptor 2/3, Metabotropic glutamate receptor 3, mGlu 2, mGlu 3, mGlu2
Presentation Serum
Reactivity Ms, Rt
Applications C, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

NGREVVDSTTSSL Corresponding to the carboxy-terminus of Rat mGLuR2
Application Immunohistochemistry: 1/30-1/100.
Immunocytochemistry: 1/30-1/100.
Western Blot: 1/250-1/500.
See Protocols for more details.
Background Metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors activated by glutamate. Based on sequence similarity, transduction mechanisms and agonist potencies, mGluRs are subdivided into three groups: mGluR1/mGluR5, mGluR2/mGluR3, and mGluR4/mGluR6/mGluR7/mGluR8. mGluRs are widely distributed throughout the nervous system and are expressed by both neurons and glial cells. mGluRs have been suggested to play a variety of functional roles, among which is involvement in synaptic plasticity underlying learning and memory as well as chronic pain.
Caution Sodium Azide interferes with peroxidase reactions and should not be used with peroxidase methodologies. If Sodium Azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with Sodium Azide-free buffer before performing the peroxidase reaction.
Protocols Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion:
1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.

Slide-mounted tissue sections were processed for Indirect Immunofluorescence.
Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
General Readings
  1. Tanabe, Y., Masu, M., Ishii, T., Shigemoto, R., and Nakanishi, S. (1992). A family of metabotropic glutamate receptors. Neuron 8 (1), 169-79.
Storage Store the antibody undiluted (in aliquots) at -20°C
Serum may have become trapped in top of vial during shipping.
Centrifugation of vial is recommended before opening.
Avoid repeated freezing and thawing.
Shelf life: 6 months from despatch.
Buffer System:
Tris Glycine, pH 7.8 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
Liquid Whole Serum
This antibody recognizes a conserved sequenece in both Metabotropic Glutamate Receptor 2 and Metabotropic Glutamate Receptor 3.
Rat, Mouse.

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