discontinued

DM3132 MGMT (O6-Methylguanin -DNA -Methyltransferase) antibody

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0.1 mg / €280.00

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Mouse anti Human MGMT (O6-Methylguanin -DNA -Methyltransferase) MT3.1

DM3132

Product Description for MGMT (O6-Methylguanin -DNA -Methyltransferase)

Mouse anti Human MGMT (O6-Methylguanin -DNA -Methyltransferase) MT3.1.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for MGMT (O6-Methylguanin -DNA -Methyltransferase)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3144
Presentation Purified
Reactivity Hu
Applications C, F, IP, P, WB
Clonality Monoclonal
Clone MT3.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Purified recombinant human MGMT protein
Mol. Wt. of antigen: 25 kD
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry
Western Blotting ( 0.5-1µg/ml for 2 hrs at RT)
Immunohistology (Formalin/paraffin, 2-4µg/ml for 30 min at RT)
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0.
Recommended positive control: Raji Cells, Colon Carcinoma.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Harris LC, et. al. Cancer Res, 1992, 52(22):6404-6.
  2. von Wronski MA, et. al. Carcinogenesis, 1994, 15:577-82.
  3. Kokkinakis DM; et al. Brit J Cancer, 1997, 75:779-88.
  4. Harris LC; et al. Carcinogen
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Purified
Specificity
Specificity:
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. Reacts with Human. Also reacts weakly with Mouse, Rat, and Hamster. Others not tested. Cellular Localization: cytoplasmic, nuclear.

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