AM33034PU-N MHC Class I Heavy Chain (Restricted expression) antibody

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0.1 mg / €320.00
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Quick Overview

Mouse anti Human MHC Class I HCA2

AM33034PU-N

Product Description for MHC Class I

Mouse anti Human MHC Class I HCA2.
Properties: Heavy Chain (Restricted expression)
Presentation: Purified
Product is tested for Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Frozen Sections, Flow Cytometry.

Properties for MHC Class I

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
Presentation Purified
Reactivity Hu
Applications C, E, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone HCA2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
Property Heavy Chain, Restricted expression
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA. 
Western Blot.
Immunoprecipitation.
Flow Cytometry. 
Immunocytochemistry.
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections
(See References for Conditions).
Recommended Dilutions: 1/100–1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
Background The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40–45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
Concentration 1.0 mg/ml
General Readings
  1. Stam NJ, Vroom TM, Peters PJ, Pastoors EB, Ploegh HL. HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. Int Immunol. 1990;2(2):113-25. PubMed PMID: 2088481.
  2. de Kruijf EM, van Nes JG, Sajet A, Tummers QR, Putter H, Osanto S, et al. The predictive value of HLA class I tumor cell expression and presence of intratumoral Tregs for chemotherapy in patients with early breast cancer. Clin Cancer Res. 2010 Feb 15;16(4):1272-80. doi: 10.1158/1078-0432.CCR-09-1844. Epub 2010 Feb 9. PubMed PMID: 20145162.
  3. Hutter H, Hammer A, Blaschitz A, Hartmann M, Ebbesen P, Dohr G, et al. Expression of HLA class I molecules in human first trimester and term placenta trophoblast. Cell Tissue Res. 1996 Dec;286(3):439-47. PubMed PMID: 8929346.
  4. Seitz C, Uchanska-Ziegler B, Zank A, Ziegler A. The monoclonal antibody HCA2 recognises a broadly shared epitope on selected classical as well as several non-classical HLA class I molecules. Mol Immunol. 1998 Sep;35(13):819-27. PubMed PMID: 9839550.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody HCA2 recognize HLA class I heavy chains. The reactivity spectrum of HCA2 is composed of all HLA-A chains (except HLA-A24), as well as some HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G chains. 
The antibody HCA2 reacts preferentially with HLA-A locus heavy chains. HCA2 was raised against free class I heavy chains of HLA, to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of Formalin-Fixed, Paraffin-Embedded Sections, and Cryo-Immuno-Electron Microscopy.
HCA2 indeed retains strong reactivity with free class I heavy chains in Western blots. HCA2 in particular reacts in a locus-specific manner by biochemical criteria.
HCA2 also produces strong reactivity in Immuno-Electron Microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens.

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