AM33374PU-N MHC Class I antibody

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0.5 ml / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human MHC Class I BRA-23/9


Product Description for MHC Class I

Mouse anti Human MHC Class I BRA-23/9.
Presentation: Purified
Product is tested for Flow Cytometry, Frozen Sections.

Properties for MHC Class I

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
Presentation Purified
Reactivity Hu
Applications C, F
Clonality Monoclonal
Clone BRA-23/9
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

REH cells, a Human pre-B cell leukaemia cell line.
Isotype control AM03096PU-N
Application Flow Cytometry.
Immunohistochemistry Frozen Sections.
Background The major histocompatibility complex (MHC) is a cluster of genes that play an important role in the immune response.  In humans, this complex is referred to as the Human Leucocyte Antigen (HLA) system. There are three major HLA class I genes which are HLA-A, HLA-B and HLA-C. These are highly polymorphic and thousands of alleles are known to exist. The HLA-A, B and C gene products are heterodimeric cell surface glycoproteins which all consist of a 40-45kD MHC-encoded alpha chain and a non-covalently linked non-MHC encoded light chain (b2-microglobulin) of 12kDa. These proteins are found on the surface of almost all nucleated cells and their role is to present peptides, produced by intracellular protein degradation, to cytotoxic T lymphocytes and natural killer cells.
Concentration 0.2 mg/ml
General Readings
  1. Tanaka J, Toubai T, Tsutsumi Y, Miura Y, Kato N, Umehara S, et al. Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells. Blood. 2004 Aug 1;104(3):768-74. Epub 2004 Apr 8. PubMed PMID: 15073036.
  2. Tóth J, Kubes M. Masking of HLA class I molecules expressed on K-562 target cells can restore their susceptibility to NK cell cytolysis. Immunobiology. 1993 Jun;188(1-2):134-44. PubMed PMID: 8406556.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0.02% Sodium Azide
0.2% BSA, 50% Glycerol
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
The antibody BRA-23/9 is directed against a non-polymorphic determinant of Human HLA Class I.
The antibody has not been tested for cross reactivity with other species. 
The BRA-23/9 antibody is suitable for the detection of HLA Class I antigen by Flow Cytometry and Immunohistochemistry on Frozen tissues, for example from human tonsil, lymph nodes, as well as lymphocytes.

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