BM4093 MHC Class I H-2 (b,d,q,h4,m,w16) antibody

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0.1 mg / €500.00
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Quick Overview

Rat anti Mouse MHC Class I H-2 ER-HR52

Product Description for MHC Class I H-2

Rat anti Mouse MHC Class I H-2 ER-HR52.
Properties: (b,d,q,h4,m,w16)
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for MHC Class I H-2

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone ER-HR52
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mouse macrophage precursor cells
MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta2-microglobulin (11.5 kDa).
The epitope recognized by ER-HR52 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.
Property b,d,q,h4,m,w16
Isotype control SM15P, SM15PX
Application Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).
Recommended Positive Control: Mouse Spleen.
Does not react on routinely processed Paraffin Sections.
Has been described to work in FACS.
Concentration 0.2 mg/ml
Protocols Protocol with Frozen, ice-cold Acetone-Fixed Sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer’s hemalum.
General Readings
  1. Klein: Natural history of the major histocompatibility complex. Wiley, New York (1986).
Storage Store the original vial at 2-8°C and the reconstituted stock solution (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography.
Buffer System:
PBS, pH 7.2 containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
Lyophilized purified IgG fraction
Restore by adding 0.5 ml distilled water.
ER-HR52 is useful for detecting MHC class I antigens. It is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
The antigen is expressed by all somatic cells at varying levels. ER-HR52 detects MHC class I antigens of various haplotypes (see below).
The antigen is found on all somatic cells in all organs sections though at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression.

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