discontinued

SM072P MHC Class I H-2Dk antibody

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0.2 mg / €370.00

Quick Overview

Mouse anti Mouse MHC Class I H-2Dk CTDk

SM072P

Product Description for MHC Class I H-2Dk

Mouse anti Mouse MHC Class I H-2Dk CTDk.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for MHC Class I H-2Dk

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CTDk
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM13P
Application Flow Cytometry (see protocol).
Concentration 0.2 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e cells, representing 1 test).
4. To each tube, add ~1.0 µg of antibody.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody FITC Goat anti-mouse IgM (H+L) at recommended dilution.
9. Incubate the tubes at 4°C for 30-60 minutes.
(It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C in media B.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide
(100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
General Readings
  1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980 Feb;124(2):533-40. PubMed PMID: 7188699.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS and 0.09% NaN3
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for cells expressing the MHC class I, H-2D antigen coded for by the k haplotype.
The reaction pattern of this antibody with a panel of in bred and recombinant haplotypes demonstrates that the antibody detects a private determinant of the H-2Dk antigen. It can be used to quantitate cells bearing the H-2Dk antigen from the appropriate strains of mice (i.e. AKR, C3H/He). Class I antigens are expressed on all nucleated cells, platelets and erythrocytes.
Species:
Mouse.

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