discontinued

BM2243F MHC Class I H-2Kd,Dd,q,u antibody

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Mouse anti Mouse MHC Class I H-2Kd,Dd,q,u A5A1-Do4

BM2243F

Product Description for MHC Class I H-2Kd,Dd,q,u

Mouse anti Mouse MHC Class I H-2Kd,Dd,q,u A5A1-Do4.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for MHC Class I H-2Kd,Dd,q,u

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms Class I major histocompatibility antigen H 2Kd
Presentation FITC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone A5A1-Do4
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Cell Surface Analysis.
Genetic Mapping.
Allele specific cell elimination.
FACS analysis: Use 1 µg to stain 1x10e6 cells in Flow Cytometry.
Complement-dependent microcytoxicity killing.
Immunoprecipitation.
Functional Studies: blocking CTLs (class I-specific).
Background MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The ''classical'' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Concentration 0.5 mg/ml (OD280 nm)
General Readings
  1. Hauptfeld, V. (1984), Immunogenetics, 19: 169.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein
FITC
Purification:
Protein A Chromatography.
Buffer System:
0.01 M PBS pH 7.2, containing 2.0 mM EDTA, 1% BSA as stabilizer and 0.09% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
This antibody detects classical mouse class I antigen; MW 45 kDa ubiquitously distributed.
Present on 100% of T-Cells, B-Cells, Erythrocytes, Macrophages, Endothelial and Epithelial tissue.
Positive with this antibody are strains of independent haplotypes H-2KdDd,q,u.
Positive are intra-H-2 recombinants carrying alleles Kd, Dd, Ld, Dq.
Strains carrying all other alleles are negative.

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