SM504PS MHC Class I (monomorphic) antibody

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0.1 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Equine MHC Class I CVS22

Product Description for MHC Class I

Mouse anti Equine MHC Class I CVS22.
Properties: (monomorphic)
Presentation: Purified
Product is tested for Immunoprecipitation, Frozen Sections, Flow Cytometry.

Properties for MHC Class I

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
Presentation Purified
Reactivity Eq
Applications C, F, IP
Clonality Monoclonal
Clone CVS22
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Equine leucocytes
Remarks:
Spleen cells from immunised mice were fused with cells of the X63.Ag 8.653 mouse myeloma cell line.
Property monomorphic
Isotype control AM03096PU-N
Application Flow cytometry: 1/25-1/200, use 10µl of this working dilution to label 10e6 cells in 100µl. 
Immunohistochemistry on frozen sections.
Immunoprecipitation.
Background MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells(). Cell Med. 2012;4(1):1-11. Epub 2012 Jan 1. PubMed PMID: 23152950. (Free PMC Article available, 6 images available)
General Readings
  1. O'Bren (1993) Ph D Thesis, University of Cambridge.
  2. Kydd, J.H. and Antczak, D.F. (1991) First International Workshop on Equine Leucocyte Antigens 12th-13th July. Preliminary Report. Equine Immunol. 4 - 5.
  3. Lunn DP, Holmes MA, Antczak DF, Agerwal N, Baker J, Bendali-Ahcene S, et al. Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 1998 Mar 31;62(2):101-43. PubMed PMID: 9638857.
  4. Mérant C, Breathnach CC, Kohler K, Rashid C, Van Meter P, Horohov DW. Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity. Vet Immunol Immunopathol. 2009 Sep 15;131(1-2):1-8. doi: 10.1016/j.vetimm.2009.03.002. Epub 2009 Mar 14. PubMed PMID: 19349079.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium azide
State:
Liquid purified IgG
Purified
Specificity
Specificity:
All tissues
Species:
Equine (Horse).

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