SM2032F MHC Class II DQ/DR (polymorphic) antibody

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Mouse anti Sheep MHC Class II DQ/DR 28.1

Product Description for MHC Class II DQ/DR

Mouse anti Sheep MHC Class II DQ/DR 28.1.
Properties: (polymorphic)
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for MHC Class II DQ/DR

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Sh
Applications F
Clonality Monoclonal
Clone 28.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ovine alveolar macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Property polymorphic
Application Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Background Ovine MHC class II antigens are expressed on several cell types, including B cells, activated T cells, monocytes, macrophages and dendritic cells.
Concentration 0.1 mg/ml
General Readings
  1. Puri NK, Mackay CR, Brandon MR. Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules. Immunology. 1985 Dec;56(4):725-33. PubMed PMID: 3908294. (Free PMC Article available)
  2. Puri NK, Gogolin-Ewens KJ, Brandon MR. Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules. Vet Immunol Immunopathol. 1987 May;15(1-2):59-86. PubMed PMID: 3303652.
  3. Puri NK, Brandon MR. Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules. Immunology. 1987 Dec;62(4):575-80. PubMed PMID: 3480873. (Free PMC Article available)
  4. Puri, N. et al. (1987) Sheep MHC class II molecules II. Identification and characterization of sheep MHC class II molecules. Immunology. 62: 575 - 580.
  5. Puri NK, de Kretser T, Brandon MR. Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products. Hum Immunol. 1987 Nov;20(3):195-207. PubMed PMID: 3501780.
  6. Sainte-Marie, G. et al. (1962) A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem. 10: 250
  7. Ballingall KT, Dutia BM, Hopkins J, Wright H. Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants. Anim Genet. 1995 Apr;26(2):79-84. PubMed PMID: 7733511.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Fluorescein Isothiocyanate Isomer 1
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Liquid purified IgG
This antibody recognises a polymorphic epitope on MHC class II DQ and DR molecules. In recent work, this clone was found to recognise MHC II transfectants DQ-T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1 (Ballingall.k. etal. 1995).

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