SM2032R MHC Class II DQ/DR (polymorphic) antibody

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100 Tests / €610.00
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Quick Overview

Mouse anti Sheep MHC Class II DQ/DR 28.1

Product Description for MHC Class II DQ/DR

Mouse anti Sheep MHC Class II DQ/DR 28.1.
Properties: (polymorphic)
Presentation: PE
Product is tested for Flow Cytometry.

Properties for MHC Class II DQ/DR

Product Category Primary Antibodies
Quantity 100 Tests
Presentation PE
Reactivity Sh
Applications F
Clonality Monoclonal
Clone 28.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Ovine alveolar macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Property polymorphic
Application Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Background Ovine MHC class II antigens are expressed on several cell types, including B cells, activated T cells, monocytes, macrophages and dendritic cells.
General Readings
  1. Puri, N. et al. (1985) Sheep lymphocyte antigens (OLA) II. Major histocompatibility complex class II molecules.Immunology. 56: 725 - 733.
  2. Puri NK, Gogolin-Ewens KJ, Brandon MR. Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules. Vet Immunol Immunopathol. 1987 May;15(1-2):59-86. PubMed PMID: 3303652.
  3. Puri NK, Brandon MR. Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules. Immunology. 1987 Dec;62(4):575-80. PubMed PMID: 3480873. (Free PMC Article available)
  4. Puri, N. et al. (1987) Sheep MHC class II molecules II. Identification and characterization of sheep MHC class II molecules. Immunology. 62: 575 - 580.
  5. Puri NK, de Kretser T, Brandon MR. Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products. Hum Immunol. 1987 Nov;20(3):195-207. PubMed PMID: 3501780.
  6. Sainte-Marie, G. et al. (1962) A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem. 10: 250
  7. Ballingall KT, Dutia BM, Hopkins J, Wright H. Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants. Anim Genet. 1995 Apr;26(2):79-84. PubMed PMID: 7733511.
Storage Prior to and following reconstitution store the antibody at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Lyophilized purified IgG
Reconstitution:
Restore with 1 ml distilled water
Specificity
Specificity:
This antibody recognises a polymorphic epitope on MHC class II DQ and DR molecules. In recent work, this clone was found to recognise ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. (Ballingall, K. et al. 1995).
Species:
Sheep.

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