SM2050P MHC Class II DQ (monomorphic) antibody

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Mouse anti Sheep MHC Class II DQ 38.27


Product Description for MHC Class II DQ

Mouse anti Sheep MHC Class II DQ 38.27.
Properties: (monomorphic)
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for MHC Class II DQ

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Reactivity Sh
Applications C, F, IP
Clonality Monoclonal
Clone 38.27
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ovine efferent duct lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Property monomorphic
Isotype control AM03095PU-N
Application Immunoprecipitation.
Immunohistochemistry on frozen sections.
Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Background The distribution of ovine DQ molecules on T lymphocytes has been shown to differ with immune status and age. Expression of DQ is upregulated after recent activation.
Concentration 1.0 mg/ml
General Readings
  1. Puri NK, Mackay CR, Brandon MR. Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules. Immunology. 1985 Dec;56(4):725-33. PubMed PMID: 3908294. (Free PMC Article available)
  2. Puri NK, Gogolin-Ewens KJ, Brandon MR. Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules. Vet Immunol Immunopathol. 1987 May;15(1-2):59-86. PubMed PMID: 3303652.
  3. Sainte Marie, G. et al. (1962) A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem. 10: 250
  4. Puri NK, Brandon MR. Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules. Immunology. 1987 Dec;62(4):575-80. PubMed PMID: 3480873. (Free PMC Article available)
  5. Puri NK, de Kretser T, Brandon MR. Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products. Hum Immunol. 1987 Nov;20(3):195-207. PubMed PMID: 3501780.
  6. Ballingall KT, Dutia BM, Hopkins J, Wright H. Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants. Anim Genet. 1995 Apr;26(2):79-84. PubMed PMID: 7733511.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
Liquid purified IgG
This antibody recognises a monomorphic epitope on MHC class II DQ molecules. This clone has been reported to react with paraffin-embedded material following alcohol fixation. Please refer to reference Sainte Marie et al for information about this protocol.
Sheep, Bovine, Goat.

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