NB100-77508 MHC Class II (I-A, I-E) antibody

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Rat anti Mouse MHC Class II (I-A, I-E) M5/114.15.2

Product Description for MHC Class II (I-A, I-E)

Rat anti Mouse MHC Class II (I-A, I-E) M5/114.15.2.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections.

Properties for MHC Class II (I-A, I-E)

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Aff - Purified
Reactivity Ms
Applications C, F, ICC/IF, IP, P
Clonality Monoclonal
Clone M5/114.15.2
Host Rat
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Activated C57BL/6 mouse spleen cells
Application flow cytometry / FACS analysis, Immunocytochemistry, Immunohistochemistry-Frozen, immunoprecipitation, Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application., flow cytometry / FACS analysis , immunoprecipitation , Immunocytochemistry , Immunohistochemistry-Frozen
Background The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2p,r haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation.
General Readings
  1. Watts, C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer, E., et al., 1998. Ann. Rev. Immunol. 16:323.
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
0.09% Sodium Azide
Immunogen affinity purified
Aff - Purified
I-A/I-E (M5/114.15.2)

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