BM4094 MHC Class II (I-A k,b,d,q,r) antibody

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0.15 mg / €460.00
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Rat anti Mouse MHC Class II (I-A k,b,d,q,r) ER-TR3

BM4094

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  • BM4094

Product Description for MHC Class II (I-A k,b,d,q,r)

Rat anti Mouse MHC Class II (I-A k,b,d,q,r) ER-TR3.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for MHC Class II (I-A k,b,d,q,r)

Product Category Primary Antibodies
Quantity 0.15 mg
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone ER-TR3
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Murine thymic reticulum.
Remarks:
Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa).
The epitope has not been further characterized.
Isotype control SM19P
Application Immunohistochemistry on Frozen Sections: 1.5 µg/ml (1/200).
Suggested Positive Control: Mouse spleen.
Does not react on routinely processed paraffin sections.
Has been reported to work in Flow Cytometry.
Background MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Concentration 0.3 mg/ml
Protocols Protocol with Frozen, ice-cold Acetone-Fixed Sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum
General Readings
  1. Van Vliet E, Melis M, Van Ewijk W. Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol. 1984 Jun;14(6):524-9. PubMed PMID: 6734714.
  2. Van Vliet E, Jenkinson EJ, Kingston R, Owen JJ, Van Ewijk W. Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur J Immunol. 1985 Jul;15(7):675-81. PubMed PMID: 4007044.
Storage Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
Stock Solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore with 0.5 ml distilled water.
Species Reactivity
Species reactivity (tested):
Mouse (Cells expressing MHC class II antigens).Does not react with Human.
Specificity
Specificity:
Monoclonal antibody ER-TR3 detects MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. it is a valuable tool for studying T-helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). This antibody also offers new possibilities for studying the development of T-helper cells since it also stains stromal cells in the thymus.
Antigen Distribution
Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages.
Tissue sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.

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