BM4094F MHC Class II (I-A k,b,d,q,r) antibody

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0.1 mg / €480.00
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Rat anti Mouse MHC Class II (I-A k,b,d,q,r) ER-TR3

Product Description for MHC Class II (I-A k,b,d,q,r)

Rat anti Mouse MHC Class II (I-A k,b,d,q,r) ER-TR3.
Presentation: FITC
Product is tested for Frozen Sections, Flow Cytometry.

Properties for MHC Class II (I-A k,b,d,q,r)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone ER-TR3
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Murine thymic reticulum.
Remarks:
Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa).
The epitope has not been further characterized.
Application Flow Cytometry: 10 µg/ml (Use 10 µl of 1/10 diluted antibody to label 10e6 cells).
Suggested Positive Control: mouse spleen.
Has beed described to work in FACS.
Background MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Concentration 0.1 mg/ml
General Readings
  1. Van Vliet E, Melis M, Van Ewijk W. Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol. 1984 Jun;14(6):524-9. PubMed PMID: 6734714.
  2. Van Vliet E, Jenkinson EJ, Kingston R, Owen JJ, Van Ewijk W. Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur J Immunol. 1985 Jul;15(7):675-81. PubMed PMID: 4007044.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: Six months from despatch.
Format
Label:
FITC
Purification:
Affinity Chromatography.
Buffer System:
Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative
State:
Liquid purified Ig fraction.
Species Reactivity
Species reactivity (tested):
Mouse (Cells expressing MHC class II antigens.Does not work in Human.
Specificity
Specificity:
This antibody is specific for cells expressing MHC class II antigens.
Antigen, epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kD) and one beta-chain (26-29 kD).
Antigen Distribution:
Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages. The level of antigen detected by ER-TR1, ER-TR 2 and ER-TR 3 differs from strain to strain (see table below).
Tissue Sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
Monoclonal antibody ER-TR 3 is one member of a family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) which detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. They are valuable tools for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). These antibodies also offer new possibilities for studying the development of T helper cells since they also stain stromal cells in the thymus.

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