BM4095 MHC Class II (I-A k,s,r) antibody

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0.1 mg / €390.00
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Quick Overview

Rat anti Mouse MHC Class II (I-A k,s,r) ER-TR2

Product Description for MHC Class II (I-A k,s,r)

Rat anti Mouse MHC Class II (I-A k,s,r) ER-TR2.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry.

Properties for MHC Class II (I-A k,s,r)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone ER-TR2
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Murine thymic reticulum
Remarks: MHC Class II antigens are heterodimers consisting of one α-chain (31-34kDa) and one β-chain (26-29kDa). The epitope has not been further characterized.
Application Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200). 
Suggested Positive Control: Mouse spleen.
Has been described to work in FACS.
Does not react on routinely processed paraffin sections.
Concentration 0.2 mg/ml (after reconstitution)
Protocols Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum
General Readings Van Vliet, E., et al.: Monoclonal Antibodies to Stromal Cell Types of the Mouse Thymus. Eur. J. Immunol. 14: 524-529 (1984)
Van Vliet, E., et al.: Stromal Cell Types in the Developing Thymus of the Normal and Nude Mouse Embryo. Eur. J. Immunol. 15: 675-681 (1985)
Storage Store lyophilized product at 2-8 °C.
Following reconstitution store (in aliqouts) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Affinity Chromatography
Buffer System:
Phosphate buffered saline, pH 7.2 (PBS)
0.01% Thimerosal
10 mg/ml BSA
Lyophilized purified Ig fraction
Restore with 0.5 ml distilled water (=stock solution).
This antibody detects cells expressing MHC class II antigens.
Monoclonal antibody ER-TR2 is one member of a family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) which detect MHC class II antigens encoded by the murine Ia region of the H-2 complex. They are valuable tools for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). These antibodies also offer new possibilities for studying the development of T helper cells since they also stain stromal cells in the thymus.
Isolated cells: The antigen is found on dendritic cells, B-cells and macrophages. The level of antigen detected by ER-TR1, ER-TR2 and ER-TR3 differs from strain to strain (see table on back).
Tissue Sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
Mouse. Does not work in Human.

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