CL090R MHC Class II I-Ad antibody

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50 µg / €260.00
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Mouse anti Mouse MHC Class II I-Ad 34-5-3S

Product Description for MHC Class II I-Ad

Mouse anti Mouse MHC Class II I-Ad 34-5-3S.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for MHC Class II I-Ad

Product Category Primary Antibodies
Quantity 50 µg
Presentation PE
Reactivity Ms
Applications F
Clonality Monoclonal
Clone 34-5-3S
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
BDF splenocytes
Remarks:
R-Phycoerythrin conjugates are produced under license and protected under Stanford University held patents 4,520,110; 4,542,104; 4,859,582; 5,055,556 (U.S.): 76695 (EPC): 548440 (Australia): 1,179,942
(Canada): and 1,594,827 (Japan).
Application Flow cytometry.
Concentration 0.1 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.2 - 0.1 μg of CL090R per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl ofepropidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
General Readings 1. Ozato, K. et al. 1982. Monoclonal Antibodies to Mouse Major Histocompatibility Complex Antigens.
Transplantation. 34: 113-120.
2. Ahn, H.J. et al. 1997. A Mechanism Underlying Synergy Between IL-12 and IFN-g-Inducing Factor in Enhanced Production of IFN-g. Journal of Immunology. 159: 2125-2131.
Storage Store at 2-8°C. DO NOT FREEZE. Avoid prolonged exposure to light.
Shelf life: one year from despatch.
Format
Label:
R-Phycoerythrin
PE
Purification:
Protein G affinity chromatography
Buffer System:
PBS with 0.02% sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
State:
Liquid Ig raction
Specificity
Specificity:
This antibody is a cytotoxic monoclonal antibody specific for cells expressing the Ia antigen coded for by the A subregion of the d, b, p, and q haplotypes (ie. I-Ad,b,p,q).
Results of flow cytometric analysis
(Tissue distribution):
Mouse Strain: BALB/c
Cell concentration : 1x10e6 cells per test
Antibody concentration used: 0.1 μg/10e6 cells
Isotypic control: PE Mouse IgG2a
Cell source percentage of cells stained above control:
Spleen 52.0% (see picture below)
Lymph Node 13.5%
(Strain distribution ):
Antibody concentration: 0.2 μg/10e6 cells
Strains Tested: A.TH, A.TL, C3H/He, C57BL/6, DBA/1
Positive: C57BL/6, DBA/1
Negative: A.TH, A.TL, C3H/He

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