NB100-77545 MHC Class II (I-Ak) antibody

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Mouse anti Mouse MHC Class II (I-Ak) 10-3.6

Product Description for MHC Class II (I-Ak)

Mouse anti Mouse MHC Class II (I-Ak) 10-3.6.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunoprecipitation, Paraffin Sections.

Properties for MHC Class II (I-Ak)

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Aff - Purified
Reactivity Ms
Applications C, F, IP, P
Clonality Monoclonal
Clone 10-3.6
Host Mouse
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

C3H mouse splenocytes
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, immunoprecipitation, Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one millioncells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application., flow cytometry / FACS analysis , immunoprecipitation , Immunohistochemistry-Frozen
Background The 10-3.6 antibody reacts with the beta chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens; does not react with other haplotypes (e.g., b, d, p, q).
General Readings
  1. Watts, C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer, E., et al., 1998. Ann. Rev. Immunol. 16:323.
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
0.09% Sodium Azide
Immunogen affinity purified
Aff - Purified
I-Ak (10-3.6)

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