discontinued

NB110-1821 MHC Class II Ia antibody

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0.1 mg / €310.00

Quick Overview

Mouse anti Mouse, Rat MHC Class II Ia OX6

NB110-1821

Product Description for MHC Class II Ia

Mouse anti Mouse, Rat MHC Class II Ia OX6.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for MHC Class II Ia

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ms, Rt
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone OX6
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Rat thymocyte membrane glycoprotein
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, IHC: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH6., flow cytometry / FACS analysis 1/50 - 1/100, Immunohistochemistry-Paraffin 1/50 1/100, Immunohistochemistry-Frozen Use at an assay dependent dilution
Background Can be used for the purification of Ia antigen from rat spleen.
Concentration 1 mg/ml
General Readings
  1. McMaster WR, Williams AF. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol. 1979 Jun;9(6):426-33. PubMed PMID: 315315.
  2. Fernandez JL, Weeks M. Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens. Lab Anim. 1986 Oct;20(4):293-7. PubMed PMID: 2430136.
  3. Charteris DG, Lightman SL. In vivo lymphokine production in experimental autoimmune uveoretinitis. Immunology. 1993 Mar;78(3):387-92. PubMed PMID: 8478022. (Free PMC Article available)
  4. Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar;43(3):313-20. PubMed PMID: 7868861.
  5. McKechnie NM, Gürr W, Braun G. Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia. J Infect Dis. 1997 Nov;176(5):1334-43. PubMed PMID: 9359736.
  6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1. B (I-A) expression by the BDIX rat strain. J. Immunogenet. 14(6):301-12
  7. Burrows GG, Bebo BF, Adlard KL, Vandenbark AA, Offner H. Two-domain MHC class II molecules form stable complexes with myelin basic protein 69-89 peptide that detect and inhibit rat encephalitogenic T cells and treat experimental autoimmune encephalomyelitis. J Immunol. 1998 Dec 1;161(11):5987-96. PubMed PMID: 9834080.
Format
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
NB110-1821 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region (1). The clone does not react with the rat BDIX strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
Species:
Mouse, Rat (-)

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