NB100-64958 MHC Class II antibody

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0.25 mg / €430.00

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Rat anti Mouse MHC Class II ER-TR1


Product Description for MHC Class II

Rat anti Mouse MHC Class II ER-TR1.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for MHC Class II

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Reactivity Ms
Applications C, F, P
Clonality Monoclonal
Clone ER-TR1
Host Rat
Isotype IgG2c
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Isolated C3H thymic stromal cells
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, This antibody is useful for Immunohistochemistry-Frozen, flow cytometry / FACS analysis, flow cytometry / FACS analysis 1/10 - 1/50, Immunohistochemistry-Frozen
Background MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Concentration 1 mg/ml
General Readings
  1. Huiskamp R, van Vliet E, van Ewijk W. Repopulation of the mouse thymus after sublethal fission neutron irradiation. II. Sequential changes in the thymic microenvironment. J Immunol. 1985 Apr;134(4):2170-8. PubMed PMID: 3882829.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.09% Sodium Azide
protein G purified
NB100-64958 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class II molecule. In the mouse, MHC class II molecules are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR1 stains cortical and medullary stromal cells. Clone ER-TR1 specifically recognizes mouse strains with the haplotypes I-A k,d,q,r. Mouse strains with the haplotypes I-A b,s,f are not recognized by this antibody.

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