NB100-64956 MHC Class II RT1B (positive on a, I, n haplotype) antibody

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Mouse anti Rat MHC Class II RT1B F17-23-2


Product Description for MHC Class II RT1B

Mouse anti Rat MHC Class II RT1B F17-23-2.
Properties: (positive on a, I, n haplotype)
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for MHC Class II RT1B

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Reactivity Rt
Applications C, F, P
Clonality Monoclonal
Clone F17-23-2
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Partially purified rat MHC antigens
Property positive on a, I, n haplotype
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, This antibody is useful for Immunohistochemistry-Frozen, flow cytometry / FACS analysis, flow cytometry / FACS analysis 1/100 - 1/200, Immunohistochemistry-Frozen
Background MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Concentration 1 mg/ml
General Readings
  1. Hart, D. N. J. and Fabre, J. W. (1981) Localisation of MHC antigens in long-surviving rat renal allografts: probable implication of the passenger leucocyte in graft adaptation. Transplantation Proceedings 13: 95-99.
  2. Hart, D. N. J. and Fabre, J. W. (1981) MHC antigens in rat kidney, ureter and bladder; localisation with monoclonal antibodies and demonstration of la positive dendritic cells. Transplantation 31: 318-325.
  3. Stet, R. J. M. et al. (1987) Effect of Haplo-specific anti class I and II monoclonal antibodies on the mitogenic and allogeneic response in rat. Transplantation proceedings. 19(3):3080-3081.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.09% Sodium Azide
protein G purified
NB100-64956 recognises the rat RT1B MHC class II antigen, reacting with haplotypes a, l and n expressed on rat strains including DA, LEW, and BN. This product is routinely tested in flow cytometry on DA rat splenocytes.

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