SM315F MHC Class II RT1B (positive on a, I, n haplotype) antibody

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0.1 mg / €430.00
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Quick Overview

Mouse anti Rat MHC Class II RT1B F17-23-2

SM315F

Product Description for MHC Class II RT1B

Mouse anti Rat MHC Class II RT1B F17-23-2.
Properties: (positive on a, I, n haplotype)
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for MHC Class II RT1B

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Rt
Applications F
Clonality Monoclonal
Clone F17-23-2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Partially purified rat MHC antigens.
Spleen cells of immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Property positive on a, I, n haplotype
Isotype control SM20F (for use in rat samples)
Application Flow Cytometry (Neat-1/5): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Concentration 0.1 mg/ml
General Readings
  1. Hart, D. N. J. and Fabre, J. W. (1981) Localisation of MHC antigens in long-surviving rat renal allografts: probable implication of the passenger leucocyte in graft adaptation. Transplantation Proceedings 13: 95 - 99.
  2. Hart, D. N. J. and Fabre, J. W. (1981) MHC antigens in rat kidney, ureter and bladder; localisation with monoclonal antibodies and demonstration of la positive dendritic cells. Transplantation 31: 318 - 325.
  3. Stet, R. J. M. et al. (1987) Effect of Haplo-specific anti class I and II monoclonal antibodies on the mitogenic and allogeneic response in rat. Transplantation proceedings. 19(3):3080-3081.
Storage Store the antibody undilued at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G.
Buffer System:
Phosphate buffered saline pH7.4 0.09% Sodium Azide, 1% Bovine Serum Albumin,
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Liquid purified IgG fraction.
Specificity
Specificity:
This antibody recognises the rat RT1B MHC class II antigen, reacting with haplotypes a, l and n expressed on rat strains including DA, LEW, and BN.
Species:
Rat.

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