SM505F MHC Class II (monomorphic) antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Equine MHC Class II CVS20

Product Description for MHC Class II

Mouse anti Equine MHC Class II CVS20.
Properties: (monomorphic)
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for MHC Class II

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HLA Class II, Human Leukocyte antigen class II, MHC Class 2, Major histocompatibility complex class II
Presentation FITC
Reactivity Eq
Applications F
Clonality Monoclonal
Clone CVS20
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
3132 cells
Remarks:
Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line.
Property monomorphic
Isotype control AM03095PU-N
Application Flow cytometry: Neat-1/10, use 10µl of this working dilution to label 10e6 cells in 100µl.
Concentration 0.1 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells(). Cell Med. 2012;4(1):1-11. Epub 2012 Jan 1. PubMed PMID: 23152950. (Free PMC Article available, 6 images available)
General Readings
  1. Kydd, J.H. & Antczak. D.F. (1991) First International Workshop on Equine Leucocyte Antigens. Equine Immunol. 4: 5.
  2. Lunn DP, Holmes MA, Antczak DF, Agerwal N, Baker J, Bendali-Ahcene S, et al. Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 1998 Mar 31;62(2):101-43. PubMed PMID: 9638857.
  3. Weiss DJ, Evanson OA, McClenahan DJ, Abrahamsen MS, Walcheck BK. Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium. Infect Immun. 2001 Feb;69(2):1002-8. PubMed PMID: 11159996. (Free PMC Article available, 7 images available)
  4. Out TA, Wang SZ, Rudolph K, Bice DE. Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs. Immunology. 2002 Apr;105(4):499-508. PubMed PMID: 11985670. (Free PMC Article available, 7 images available)
  5. Carrade DD, Owens SD, Galuppo LD, Vidal MA, Ferraro GL, Librach F, et al. Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses. Cytotherapy. 2011 Apr;13(4):419-30. doi: 10.3109/14653249.2010.536213. Epub 2010 Nov 25. PubMed PMID: 21105841.
  6. Catchpole B, Stell AJ, Dobson JM. Generation of blood-derived dendritic cells in dogs with oral malignant melanoma. J Comp Pathol. 2002 Feb-Apr;126(2-3):238-41. PubMed PMID: 11945016.
  7. Weiss DJ, Evanson OA, Souza CD. Mucosal immune response in cattle with subclinical Johne's disease. Vet Pathol. 2006 Mar;43(2):127-35. PubMed PMID: 16537930.
  8. Weiss DJ. Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies. Vet Pathol. 2001 Sep;38(5):512-8. PubMed PMID: 11572558.
  9. Sassa Y, Inoshima Y, Ishiguro N. Bovine macrophage degradation of scrapie and BSE PrPSc. Vet Immunol Immunopathol. 2010 Jan 15;133(1):33-9. doi: 10.1016/j.vetimm.2009.06.018. Epub 2009 Jul 7. PubMed PMID: 19647878.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium azide and 1% BSA
State:
Liquid purified IgG
Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Human
Species reactivity (tested):
Equine (Horse)
Specificity
Specificity:
This antibdoy reacts with MHC Class II antigen. This antibdy reacts with all equine B cells and 95% of equine T cells.

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