AM08450PU-N MHV NSP9 (Strain A59) antibody

See related secondary antibodies

Search for all "MHV NSP9"

0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse MHV NSP9 2C6.H1

AM08450PU-N

More Views

  • AM08450PU-N

Product Description for MHV NSP9

Mouse anti Mouse MHV NSP9 2C6.H1.
Properties: (Strain A59)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for MHV NSP9

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Mouse Hepatitis Virus Strain A59, Murine coronavirus, Nonstructural Protein 9
Presentation Purified
Reactivity Ms
Applications ICC/IF, WB
Clonality Monoclonal
Clone 2C6.H1
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0C6X9
Immunogen:
E. coli derived fulllength MHV-A59 nsp9 protein.
Remarks:
This protein is part of the viral replicase polyprotein.
Property Strain A59
Application Western blot: 1:1,000 (Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 in the appropriate cell lysate or extract).
Immunofluorescence: 1:1,000 (Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min).

Background The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Concentration 2.1 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Ziebuhr J, Snijder EJ, Gorbalenya AE. Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol. 2000 Apr;81(Pt 4):853-79. PubMed PMID: 10725411.
  2. Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, et al. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J Mol Biol. 2003 Aug 29;331(5):991-1004. PubMed PMID: 12927536.
  3. Egloff MP, Ferron F, Campanacci V, Longhi S, Rancurel C, Dutartre H, et al. The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world. Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3792-6. Epub 2004 Mar 8. PubMed PMID: 15007178. (Free PMC Article available, 3 images available)
  4. Sutton G, Fry E, Carter L, Sainsbury S, Walter T, Nettleship J, et al. The nsp9 replicase protein of SARS-coronavirus, structure and functional insights. Structure. 2004 Feb;12(2):341-53. PubMed PMID: 14962394.
  5. Bost AG, Carnahan RH, Lu XT, Denison MR. Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly. J Virol. 2000 Apr;74(7):3379-87. PubMed PMID: 10708455. (Free PMC Article available, 9 images available)
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Format
Purification:
Protein A chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
This antibody is directed against the MHV-A59 nsp9 protein.

Accessory Products

  • LinkedIn