DM3133 Microphthalmia (Mi-Protein, MiTF) antibody

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Mouse anti Canine, Equine, Human, Mouse, Rat Microphthalmia (Mi-Protein, MiTF) C5


Product Description for Microphthalmia (Mi-Protein, MiTF)

Mouse anti Canine, Equine, Human, Mouse, Rat Microphthalmia (Mi-Protein, MiTF) C5.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Microphthalmia (Mi-Protein, MiTF)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Can, Eq, Hu, Ms, Rt
Applications P, WB
Clonality Monoclonal
Clone C5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

N-terminal fragment of human Mi protein.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Gel Supershift (Order Ab at 1mg/ml). Western Blotting. Immunohistology (Formalin/paraffin): Use Ab at 2-4µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: 501 Mel Cells, Melanoma. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells, and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. Reacts with Human, Horse. Does not react with Mouse, and Rat. Others not tested. Cellular Localization: nuclear.

Mol. Wt. of Antigen: 52-56kD

Notes: This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. This antibody reacts with both melanocytic and nonmelanocytic isoforms of Mi. It is especially useful for research on murine Mi protein. Epitope: N-terminal

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