SM6013 MIF antibody

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0.1 ml / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human MIF 4E4

SM6013

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Product Description for MIF

Mouse anti Human MIF 4E4.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for MIF

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, P, WB
Clonality Monoclonal
Clone 4E4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14174
Immunogen:
Recombinant Human MIF (1-114 aa) purified from E. coli.
GeneID:
4282
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA.
Western blot:
1/500-1/2,000. 
Flow Cytometry. 
Immunofluorescence/Immnunocytochemistry.
Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Background The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Concentration 1.0 mg/ml
General Readings
  1. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7522-6. PubMed PMID: 2552447. (Free PMC Article available)
  2. Bernhagen J, Mitchell RA, Calandra T, Voelter W, Cerami A, Bucala R. Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). Biochemistry. 1994 Nov 29;33(47):14144-55. PubMed PMID: 7947826.
  3. Bucala R. MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response. FASEB J. 1996 Dec;10(14):1607-13. PubMed PMID: 9002552.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
The antibody recognizes Human MIF.
Other species not tested.
Gene ID 4282

Accessory Products

Proteins and/or Positive Controls

Proteins for MIF (15 products)

Catalog No. Species Pres. Purity   Source  

MIF

MIF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

MIF

MIF Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
25 µg / €139.00
  OriGene Technologies, Inc.

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.1 mg / €340.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MIF

MIF Human
  Abnova Taiwan Corp.

MIF

MIF Human Purified > 95 % E. coli
5 µg / €190.00
  Neuromics Antibodies

MIF

MIF Human Purified > 95 % E. coli
25 µg / €290.00
  Neuromics Antibodies

MIF

MIF Human Purified
  Novus Biologicals Inc.

MIF

MIF Human Purified
  Novus Biologicals Inc.

Positive controls for MIF (1 products)

Catalog No. Species Pres. Purity   Source  

MIF Lysate

Western Blot: MIF Lysate [NBL1-13108] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MIF
  Novus Biologicals Inc.
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