TA303028 MIF (C-term) antibody

Goat Polyclonal Antibody against MIF

See related secondary antibodies

Search for all "MIF"

0.1 mg / €360.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human, Mouse, Rat MIF

TA303028

More Views

  • TA303028

Product Description for MIF

Goat anti Human, Mouse, Rat MIF.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for MIF

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P14174
Immunogen:
Peptide with sequence from the C Terminus of the protein sequence according to NP_002406.1.
AA Sequence:
C-NAANVGWNNSTFA
GeneID:
4282
Property C-term
Add. information Calculated Molecular Weight: 12.5kDa (NP_002406.1).
Application Peptide ELISA: 1/128000 (Detection Limit).
Western Blot: 0.01-0.03 µg/ml. A band of Approx 13kDa is detected in Human Lymph Node and Thymus lysates.
Enzyme Immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody.
Recommended reporter concentration: 1.5-3 µg/ml.
Background MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.
Concentration 0.5 mg/ml
General Readings Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Buffer System:
Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes Macrophage migration inhibitory factor.

Accessory Products

Proteins and/or Positive Controls

Proteins for MIF (15 products)

Catalog No. Species Pres. Purity   Source  

MIF

MIF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

MIF

MIF Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
25 µg / €139.00
  OriGene Technologies, Inc.

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH

MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.1 mg / €340.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MIF

MIF Human
  Abnova Taiwan Corp.

MIF

MIF Human Purified > 95 % E. coli
5 µg / €190.00
  Neuromics Antibodies

MIF

MIF Human Purified > 95 % E. coli
25 µg / €290.00
  Neuromics Antibodies

MIF

MIF Human Purified
  Novus Biologicals Inc.

MIF

MIF Human Purified
  Novus Biologicals Inc.

Positive controls for MIF (1 products)

Catalog No. Species Pres. Purity   Source  

MIF Lysate

Western Blot: MIF Lysate [NBL1-13108] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MIF
  Novus Biologicals Inc.
  • LinkedIn