Product Description for MIF
Goat anti Human, Mouse, Rat MIF.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for MIF
Product Category | Primary Antibodies |
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Target Category | |
Quantity | 0.1 mg |
Synonyms | GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase |
Presentation | Aff - Purified |
Reactivity | Hu, Ms, Rt |
Applications | E, WB |
Clonality | Polyclonal |
Host | Goat |
Shipping to | Europe, USA/Canada |
PDF datasheet | View Datasheet |
Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
Immunogen |
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Property | C-term |
Add. information | Calculated Molecular Weight: 12.5kDa (NP_002406.1). |
Application | Peptide ELISA: 1/128000 (Detection Limit). Western Blot: 0.01-0.03 µg/ml. A band of Approx 13kDa is detected in Human Lymph Node and Thymus lysates. Enzyme Immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3 µg/ml. |
Background | MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis. |
Concentration | 0.5 mg/ml |
General Readings | Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29 |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
Format |
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Species Reactivity |
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Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for MIF (15 products)
Catalog No. | Species | Pres. | Purity | Source | |||
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TP305106 |
MIF |
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Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
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OriGene Technologies, Inc. | |||||||
TP723300 |
MIF |
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Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
25 µg /
€139.00
|
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OriGene Technologies, Inc. | |||||||
AR03003PU-N |
MIF (1-115) |
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Human | Purified | > 98 % pure by SDS PAGE | E. coli |
0.5 mg /
€990.00
|
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Acris Antibodies GmbH | |||||||
AR03003PU-S |
MIF (1-115) |
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Human | Purified | > 98 % pure by SDS PAGE | E. coli |
0.1 mg /
€340.00
|
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Acris Antibodies GmbH | |||||||
AR51921PU-N |
MIF (1-115, His-tag) |
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Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
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Acris Antibodies GmbH | |||||||
AR51921PU-S |
MIF (1-115, His-tag) |
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Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
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Acris Antibodies GmbH | |||||||
SA6053 |
MIF (1-115, His-tag) |
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Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€230.00
|
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Acris Antibodies GmbH | |||||||
SA6053X |
MIF (1-115, His-tag) |
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Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.5 mg /
€540.00
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Acris Antibodies GmbH | |||||||
H00004282-P01-10 |
MIF |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
Abnova Taiwan Corp. | |||||||
H00004282-P01-25 |
MIF |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
Abnova Taiwan Corp. | |||||||
P6067 |
MIF |
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Human | ||||||
Abnova Taiwan Corp. | |||||||
PR27042-5 |
MIF |
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Human | Purified | > 95 % | E. coli |
5 µg /
€190.00
|
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Neuromics Antibodies | |||||||
PR27042-25 |
MIF |
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Human | Purified | > 95 % | E. coli |
25 µg /
€290.00
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Neuromics Antibodies | |||||||
NBP1-43181-5 |
MIF |
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Human | Purified | |||||
Novus Biologicals Inc. | |||||||
NBP1-43181-25 |
MIF |
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Human | Purified | |||||
Novus Biologicals Inc. |
Positive controls for MIF (1 products)
Catalog No. | Species | Pres. | Purity | Source | |||
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NBL1-13108 |
MIF Lysate |
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Novus Biologicals Inc. |
Recommended Secondary Antibodies for MIF (9 products)
Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
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R1317AP |
Goat IgG (H+L chain) antibody |
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Rabbit | AP | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1317B |
Goat IgG (H+L chain) antibody |
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Rabbit | Biotin | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1317F |
Goat IgG (H+L chain) antibody |
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Rabbit | FITC | E, ICC/IF | ||||
Acris Antibodies GmbH | |||||||
R1317HRP |
Goat IgG (H+L chain) antibody |
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Rabbit | HRP | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1317T |
Goat IgG (H+L chain) antibody |
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Rabbit | TRITC | E, ICC/IF | ||||
Acris Antibodies GmbH | |||||||
R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Donkey | PE | |||||
Acris Antibodies GmbH | |||||||
R1435C2 |
Goat IgG (H+L chain), adsorbed antibody |
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Donkey | Cy2 | |||||
Acris Antibodies GmbH | |||||||
R1435C3 |
Goat IgG (H+L chain), adsorbed antibody |
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Donkey | Cy3 | |||||
Acris Antibodies GmbH | |||||||
R1435C5 |
Goat IgG (H+L chain), adsorbed antibody |
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Donkey | Cy5 | F, ICC/IF | ||||
Acris Antibodies GmbH |