MIF (C-term) - TA303028 | acris-antibodies.com

Product Description for MIF

Goat anti Human, Mouse, Rat MIF.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for MIF

Product Category	Primary Antibodies
Target Category	MIF antibody
Quantity	0.1 mg
Synonyms	GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	E, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Europe, USA/Canada
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies, Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P14174 Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002406.1. AA Sequence: `C-NAANVGWNNSTFA` GeneID: 4282
Property	C-term
Add. information	Calculated Molecular Weight: 12.5kDa (NP_002406.1).
Application	Peptide ELISA: 1/128000 (Detection Limit). Western Blot: 0.01-0.03 µg/ml. A band of Approx 13kDa is detected in Human Lymph Node and Thymus lysates. Enzyme Immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3 µg/ml.
Background	MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.
Concentration	0.5 mg/ml
General Readings	Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP. Obesity, macrophage migration inhibitory factor, and weight loss. Int J Obes Relat Metab Disord. 2005 Mar 29
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide. Buffer System: Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Liquid purified Ig fraction Aff - Purified
Species Reactivity	Species reactivity (expected): Mouse, Rat. Species reactivity (tested): Human.
Specificity	Specificity: This antibody recognizes Macrophage migration inhibitory factor.

Accessory Products

Proteins and/or Positive Controls

Proteins for MIF (15 products)

Catalog No.	Species	Pres.	Purity	Source
TP305106	MIF
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €438.00
					OriGene Technologies, Inc.
TP723300	MIF
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		25 µg / €139.00
					OriGene Technologies, Inc.
AR03003PU-N	MIF (1-115)
	Human	Purified	> 98 % pure by SDS PAGE	E. coli	0.5 mg / €990.00
					OriGene Technologies GmbH
AR03003PU-S	MIF (1-115)
	Human	Purified	> 98 % pure by SDS PAGE	E. coli	0.1 mg / €340.00
					OriGene Technologies GmbH
AR51921PU-N	MIF (1-115, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €730.00
					OriGene Technologies GmbH
AR51921PU-S	MIF (1-115, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €300.00
					OriGene Technologies GmbH
SA6053	MIF (1-115, His-tag)
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.1 mg / €230.00
					OriGene Technologies GmbH
SA6053X	MIF (1-115, His-tag)
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.5 mg / €540.00
					OriGene Technologies GmbH
H00004282-P01-10	MIF
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00004282-P01-25	MIF
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P6067	MIF
	Human
					Abnova Taiwan Corp.
PR27042-5	MIF
	Human	Purified	> 95 %	E. coli	5 µg / €190.00
					Neuromics Antibodies
PR27042-25	MIF
	Human	Purified	> 95 %	E. coli	25 µg / €290.00
					Neuromics Antibodies
NBP1-43181-5	MIF
	Human	Purified
					Novus Biologicals Inc.
NBP1-43181-25	MIF
	Human	Purified
					Novus Biologicals Inc.

Positive controls for MIF (1 products)

Catalog No.	Species
NBL1-13108	MIF Lysate

		Novus Biologicals Inc.

Recommended Secondary Antibodies for MIF (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH