AM00350PU-N Mitochondria antibody

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Mouse anti Human Mitochondria MTC02

Product Description for Mitochondria

Mouse anti Human Mitochondria MTC02.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Mitochondria

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone MTC02
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Semi-purified mitochondrial preparation.
Mol. Wt. of Antigen: 60 kDa
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunofluorescence.
Western Blotting: 1-2 micrograms/ml for 2hrs at RT.
Immunohistology (Formalin/paraffin): 1-2 micrograms/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended Positive Control: HeLa Cells, Liver.
Background This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.
Concentration 0.2 mg/ml
Storage Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography.
Buffer System:
10 mM PBS, pH 7.4, with 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Liquid purified IgG fraction.
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions.
Cellular Localization: Cytoplasmic.

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