AM33287PU-T Mitochondria antibody

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20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Mitochondria SPM198

Product Description for Mitochondria

Mouse anti Human Mitochondria SPM198.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Mitochondria

Product Category Primary Antibodies
Quantity 20 µg
Synonyms Mitochondrial Marker
Presentation Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone SPM198
Host Mouse
Isotype IgG1
Molecular weight 60kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Semi-purified mitochondrial preparation.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 0.25-0.5 µg/ml. 
Immunofluorescence
: 0.5-1 µg/ml. 
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: HeLa or HepG2 cells, Hepatic carcinoma.
Background Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998).
Concentration 0.2 mg/ml
General Readings
  1. Epstein, Alan L. and Clevenger, Charles V. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, Ed. CRC Press, Boca Raton, FL, pp. 117-137, 1985.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Cellular LocalizationMitochondria in cytoplasm. 
Negative Species: Mouse and Rat.

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