AM33293PU-N Mitochondria antibody

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0.2 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Mitochondria MTC02

Product Description for Mitochondria

Mouse anti Human Mitochondria MTC02.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Mitochondria

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone MTC02
Host Mouse
Isotype IgG1
Molecular weight 60kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Semi-purified mitochondrial preparation.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use BSA free Antibody for Coating.
Western Blot: 0.25-0.5 µg/ml. 
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. 
Immunofluorescence: 0.5-1 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: HeLa or HepG2 cells. Hepatic carcinoma.
Concentration 0.2 mg/ml
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal Antibody recognizes a 60kDa antigen associated with the m itochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular or ganelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondr ia in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mito chondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed f rozen tissue or cell preparations.
This Monoclonal Antibody is an excellent marker for human cells in xenographic mo del research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Cellular Localization: Mitochondria in cytoplasm. 
Negative Species: Mouse, Rat.

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