AM33336PU-T Mitochondria antibody

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20 µg / €180.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Mitochondria MTC719


Product Description for Mitochondria

Mouse anti Human Mitochondria MTC719.
Presentation: Purified
Product is tested for Flow Cytometry, Western blot / Immunoblot, Immunoprecipitation, Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Mitochondria

Product Category Primary Antibodies
Quantity 20 µg
Synonyms Mitochondrial Marker
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone MTC719
Host Mouse
Isotype IgG1
Molecular weight 60kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mitochondrial fraction of HeLa cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use BSA Free Antibody for coating.
Western Blot:
 0.25-0.5 µg/ml. 
Flow Cytometry:  0.5-1 µg/106 cells.
: 0.5-1 µg/ml. 
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: HeLa or HepG2 cells. Hepatic carcinomas.
Background Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998).
Concentration 0.2 mg/ml
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
Human. Shows broad species reactivity.
This Monoclonal recognizes  a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondri al marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cel ls and may be used to stain mitochondria of cells in fixed or frozen tissue sections . It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Cellular LocalizationMitochondria in cytoplasm. 
Negative Species: Insects or Bacteria.

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