AF6910 MMP-9 antibody

See related secondary antibodies

Search for all "MMP-9"

0.1 mg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human MMP-9 56-2A4

AF6910

More Views

  • AF6910

Product Description for MMP-9

Mouse anti Human MMP-9 56-2A4.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for MMP-9

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, GELB, Gelatinase B, MMP9, Matrix metalloproteinase-9
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone 56-2A4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14780
Immunogen:
Oligopeptide of RSASEVDRMFPGVPLDTHD (residue 626 - 644) on a Human matrix metalloproteinase 9 (hMMP-9, gelatinase B).
GeneID:
4318
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blotting: 2-5 µg/ml. Recognizes a band corresponding to pro-MMP9 at 92 kDa and an intermediate form at 83 kDa when evaluated versus the conditioned medium of Human fibrosarcoma (HT1080 cells).
Immunohistochemistry on Frozen Sections: 1-5 µg/ml. 
Immunohistochemistry on Paraffin Sections: 1-5 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Matrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form. MMP-9 shows substrate specificity toward type IV and V collagens, gelatin and elastin.
Concentration 0.5 mg/ml
General Readings
  1. Kawahara, E. et al., Jpn. J. Cancer Res., 84. 409-418, 1993.
  2. Fujimoto, N. et al., Clin. Chim. Acta. 221. 91-103, 1993.
  3. Nakagawa, T. et al., J. Neurosurg., 81. 69-77, 1994.
  4. Nomura, H. et al., Int. J. Cancer, 69. 9-16, 1996.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Preservatives:
None
Stabilizers:
0.5% Protease-free BSA
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Guinea Pig.
Specificity
Specificity:
The monoclonal 56-2A4 antibody specifically reacts with precursor (92 kDa) and intermediate (83 kDa) forms of Human MMP-9 and cross-reacts with Guinea Pig, Rat and Rabbit MMP-9.
Does not react with Human MMP-1, 2, 3, 13 and active Human MMP-9 (67 kDa) or Mouse, Rat and Bovine MMP-9.
Gene ID 4318
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-9 (7 products)

Catalog No. Species Pres. Purity   Source  

MMP-9

MMP-9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MMP-9

MMP-9 Human > 95 % pure by SDS-PAGE: This preparation is free from gelatinase B dimers and from complexes of gelatinase B with TIMP-1 or lipocalin. Blood
5 µg / €430.00
  Acris Antibodies GmbH

MMP-9

MMP-9 Human
2 µg / €300.00
  Abnova Taiwan Corp.

MMP-9

MMP-9 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

MMP-9

MMP-9 Human Purified
25 µg / €510.00
  Abnova Taiwan Corp.

MMP-9

MMP-9 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

MMP-9

MMP-9 Human
10 µg / €580.00
  Abnova Taiwan Corp.

Positive controls for MMP-9 (2 products)

Catalog No. Species Pres. Purity   Source  

MMP9 Lysate

Western Blot: MMP9 Lysate [NBL1-13162] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MMP9
0.1 mg / €400.00
  Novus Biologicals Inc.

MMP9 overexpression lysate

MMP9 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
  • LinkedIn