discontinued

SP6214P MTA2 / PID / MTA1L1 (652-668) antibody

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50 µg / €210.00

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Rabbit anti Human, Mouse, Rat MTA2 / PID / MTA1L1 (652-668)

SP6214P

Product Description for MTA2 / PID / MTA1L1 (652-668)

Rabbit anti Human, Mouse, Rat MTA2 / PID / MTA1L1 (652-668).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for MTA2 / PID / MTA1L1 (652-668)

Product Category Primary Antibodies
Quantity 50 µg
Synonyms MTA-2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding to amino acids 652 to 668 of human PID/MTA2 (6), which differ from the mouse sequence by one amino acid (7).

Format: This antibody is supplied as liquid purified immunglobulin fraction in Phosphate buffered saline with 0.02% sodium azide as preservative.
Application Suitable for Western blot (0.5 - 1.0 µ/ml, use HeLa cell lysate as positive control). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7. PubMed PMID: 9836640.
  2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000 Mar 2;404(6773):42-9. PubMed PMID: 10716435.
  3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000 May 12;288(5468):1053-8. PubMed PMID: 10807576.
  4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62. PubMed PMID: 11030628.
  5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis. Nat Genet. 2000 Sep;26(1):122-7. PubMed PMID: 10973264.
  6. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature. 2000 Nov 16;408(6810):377-81. PubMed PMID: 11099047.
  7. Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D. Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes Dev. 1999 Aug 1;13(15):1924-35. PubMed PMID: 10444591. (Free PMC Article available, 7 images available)
  8. Futamura M, Nishimori H, Shiratsuchi T, Saji S, Nakamura Y, Tokino T. J Hum Gen
Storage Store the antibody undiluted at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Format
State:
Aff - Purified
Specificity
Specificity:
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex
(6-8). PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6). PID/MTA2 is ubiquitously expressed in human tissues (8).
This antibody reacts with human PID/MTA2.

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