discontinued

AP31963PU-N Mucin-10 / Prol1 antibody

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0.1 mg / €340.00

Quick Overview

Goat anti Mouse Mucin-10 / Prol1

Product Description for Mucin-10 / Prol1

Goat anti Mouse Mucin-10 / Prol1.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Enzyme Immunoassay.

Properties for Mucin-10 / Prol1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Alternative namesBasic proline rich lacrimal protein, BPLP, Muc10, PRL1, Proline rich 1, submandibular gland salivary mucin
Presentation Aff - Purified
Reactivity Ms
Applications C, E, ICC/IF
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q80XS5
Immunogen:
Synthetic peptide QFPVRKYLEDPRY with a Cysteine residue linker, corresponding to internal sequence amino acids 30-42 of Mouse Prol1 (NP_032670.2).
AA Sequence:
C-QFPVRKYLEDPRY
Application
Peptide ELISA: 1/32000 (Detection limit). 
Western Blot: Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. 
Immunohistochemistry: Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland.
Data provided by winner Melinda Larsen State University of New York, Albany, NY.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Nelson DA, Manhardt C, Kamath V, Sui Y, Santamaria-Pang A, Can A, et al. Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation. Biol Open. 2013 Apr 18;2(5):439-47. doi: 10.1242/bio.20134309. Print 2013 May 15. PubMed PMID: 23789091. (Free PMC Article available, 5 images available)
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Immunoaffinity Chromatography
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
Recognizes Mucin 10 / Prol1

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