BM4077 Mucin-Like Carcinoma Antigen (MCA) antibody

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50 µg / €530.00
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Mouse anti Human Mucin-Like Carcinoma Antigen (MCA) b-12

Product Description for Mucin-Like Carcinoma Antigen (MCA)

Mouse anti Human Mucin-Like Carcinoma Antigen (MCA) b-12.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Mucin-Like Carcinoma Antigen (MCA)

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Marker for Mucin Producing Cells
Presentation Aff - Purified
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone b-12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Breast carcinoma cell lines.
Application Immunohistochemistry on:
Frozen sections: 0.5 µg/ml (1/200).
Paraffin sections: 4 µg/ml (1/25); Proteinase K pretreatment for antigen retrieval recommended.
Suggested positive control: Human uterus.
Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is only located at the MCA producing sites such as glandular cell surfaces or glandular tubuli. In MCA producing tumors, where cells become disorganized, the b-12 antigen is secreted into stromal tissue and blood vessels. (See Table 1.)
Concentration 0.1 mg/ml
Protocols Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.

Protocol with formalin-fixed, paraffin-embedded sections:
The whole procedure is performed at room temperature
1. Deparaffinize and rehydrate tissue section
2. Incubate the tissue section with proteinase K for 7 min.
3. Wash in distilled water
4. Block endogenous peroxidase
5. Wash in PBS
6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
10. Wash in PBS
11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
12. Wash in PBS
13. Counterstain with Mayer's hemalum.
General Readings
  1. Stähli C, Takacs B, Miggiano V, Staehelin T, Carmann H. Monoclonal antibodies against antigens on breast cancer cells. Experientia. 1985 Nov 15;41(11):1377-81. PubMed PMID: 2415385.
  2. Zenklusen, H.R. et al.: The immunohistochemical reactivity of a new anti-epithelial antibody (mAb b-12) against breast carcinoma and other normal and neoplastic human tissues. Virchows Arch A Pathol Anat 413: 3. (1988)
  3. Maurer, A. & Burckhardt, J.: Biochemistry and molecular biology of MCA. Int. J. Biol. Markers 8: 108-112. (1993)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
PBS, buffer pH 7.2 with 0.05% Sodium Azide as preservative and 2 mg/ml BSA as stabilizer
State:
Lyophilized purified Ig fraction.
Aff - Purified
Reconstitution:
Restore with 0.5 ml distilled water.
Specificity
Specificity:
b-12 is useful for identifying mucin-like carcinoma antigen (MCA) produced by various tumors and certain healthy glandular cells. In combination with other BMA markers for inflammation staging or investigating neo-vascularization processes b-12 is a valuable tool for studying tumor growth or regression. MCA is a 350 kDa glycoprotein with the typical biochemical characteristics of mucin-like glycoproteins (sialomucins) which protect surfaces. Antibody b-12 binds to the protein backbone of MCA, not to the large number of carbohydrate side chains. This antibody reacts with MCA producing cells.
MCA consists of a polymorphic family of glycoproteins. The b-12 related antigenic epitope is located in the more constant region of MCA.
Specificity for Human MCA producing cells.
Species:
Human: MCA producing Cells.

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