AM31836PU-N Myeloid Lineage antibody

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0.25 mg / €280.00
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Quick Overview

Mouse anti Rat Myeloid Lineage OX-82

Product Description for Myeloid Lineage

Mouse anti Rat Myeloid Lineage OX-82.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for Myeloid Lineage

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Reactivity Rt
Applications C, F, WB
Clonality Monoclonal
Clone OX-82
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Anemic Rat bone marrow.
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application Flow Cytometry (See Protocols).
This clone OX-82 has been reported for use in Western Blotting and Immunohistochemistry on Frozen Sections (Ref. 2).
Background Hematopoietic stem cells (HSC) are the precursor cells found in the bone marrow which give rise to all the blood cell types of both the Myeloid and lymphoid lineages, which include monocytes and macrophages, neutrophils, basophils, eosinophils, T cells, B cells, NK cells, microglia, erythrocytes, megakaryocytes and dendritic cells. During the process of hematopoiesis, Myeloid lineage cells originate from the bone marrow, while Lymphoid lineage cells originate from the lymph tissue. Blimp-1 is a key regulator of the differentiation of the separate hematopoietic myeloid and lymphoid lineages.
The distinction between myeloid and lymphoid lineages is essential to diagnose and treat certain cancers. Myeloid lineage cells induce inflammatory cytokine production upon activation by Kaposi's sarcoma-associated herpesvirus OX2 glycoprotein. At the stage of myelocytes, Myeloid lineage cells express a substantial number of IL-8 receptor homologs.
Concentration 1.0 mg/ml
Protocols Flow Cytometry Analysis:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add 0.1 μg of AM31836PU-N.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 μl of secondary antibody FITC Goat anti-Mouse IgG (H+L)) at 1:500 dilution.
9. Incubate the tubes at 4°C for 30-60 minutes.
(It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C in media B.
11. Resuspend the cell pellet in 50 μl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results:
Tissue Distribution By Flow Cytometry Analysis:
Rat Strain: Wistar
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.1 ug /10e6 cells
Isotypic Control: Mouse IgG1
General Readings
  1. Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. 1996;4(4):235-46. PubMed PMID: 8924759. (Free PMC Article available)
  2. Hoffmann JC, Herklotz C, Zeitz M, Bayer B, Zeidler H, Westermann J. Effects of the anti-CD2 mAb OX34 on in vivo proliferation. Ann N Y Acad Sci. 1998 Nov 17;859:216-8. PubMed PMID: 9928391.
Storage Store the antibody undiluted at 2-8°C for one month or (in alqiuots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography of Ascites fluid.
Buffer System:
PBS containing 0.02% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Purified
Species Reactivity
Species reactivity (tested):
Rat.
Specificity
Specificity:
This Myeloid Lineage monoclonal antibody recognizes a 35 kDa antigen found on myeloid cells and stromal elements from a variety of tissues in the adult Rat.

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